Gelfand D H, Hayashi M
Proc Natl Acad Sci U S A. 1970 Sep;67(1):13-7. doi: 10.1073/pnas.67.1.13.
An antibody-precipitable structural protein from the bacteriophage varphiX174 was purified from an in vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from the bacteriophage. The tryptic hydrolysate was subjected to high resolution peptide chromatography which revealed essentially identical patterns for the varphiX174 proteins synthesized in vitro and in vivo.
一种来自噬菌体φX174的可被抗体沉淀的结构蛋白,是从用该病毒的复制性DNA引发的体外蛋白质合成系统中纯化得到的。在存在从噬菌体中纯化出的差异标记的类似蛋白的情况下,对该蛋白进行胰蛋白酶消化。将胰蛋白酶水解产物进行高分辨率肽色谱分析,结果显示体外和体内合成的φX174蛋白的图谱基本相同。