左旋多巴给药后尿液中多巴胺硫酸结合物的高效液相色谱测定法。

High-performance liquid chromatographic determination of dopamine sulfoconjugates in urine after L-dopa administration.

作者信息

Arakawa Y, Imai K, Tamura Z

出版信息

J Chromatogr. 1979 Mar 1;162(3):311-8. doi: 10.1016/s0378-4347(00)81517-0.

Abstract

A procedure was developed for the separation and determination of dopamine-3-O-sulfate (DM-3-S) and dopamine-4-O-sulfate (DM-4-S) in the urine of subjects administered L-DOPA. The method consists of sample preparation using cation- and anion-exchange resins followed by determination of the sulfates by high-performance liquid chromatography. The addition recoveries were 96 +/- 2.9% (S.D.) for DM-3-S and 93 +/- 3.0% (S.D.) for DM-4-S. Twenty samples could be measured per day. When every 2-h urine specimen from normal subjects was analyzed after L-DOPA administration (0.5 g), the maximum excretion of each sulfate was observed in the second 2-h specimen. For the first 6 h 7.5 +/- 1.5% (S.D.) of the administered L-DOPA was excreted as DM-O-sulfates. During this time, the ratio of DM-4-S to the DM-O-sulfates was 11.7 +/- 0.58% (S.D.).

摘要

开发了一种用于分离和测定服用左旋多巴受试者尿液中多巴胺 - 3 - O - 硫酸盐（DM - 3 - S）和多巴胺 - 4 - O - 硫酸盐（DM - 4 - S）的方法。该方法包括使用阳离子和阴离子交换树脂进行样品制备，然后通过高效液相色谱法测定硫酸盐。DM - 3 - S的加样回收率为96±2.9%（标准差），DM - 4 - S的加样回收率为93±3.0%（标准差）。每天可测量20个样品。当给正常受试者服用左旋多巴（0.5 g）后分析每2小时的尿液标本时，在第二个2小时标本中观察到每种硫酸盐的最大排泄量。在最初6小时内，服用的左旋多巴有7.5±1.5%（标准差）以DM - O - 硫酸盐的形式排泄。在此期间，DM - 4 - S与DM - O - 硫酸盐的比例为11.7±0.58%（标准差）。