Girbardt M
J Cell Biol. 1965 Nov;27(2):433-40. doi: 10.1083/jcb.27.2.433.
By means of a special selective preparation technique, it is possible to investigate in thin sections, by electron microscopy, areas of a cell that have been observed in the living state, by phase-contrast microscopy, up to the time of fixation. Structures recorded in the living state can thus be compared to structures seen in electron micrographs. In cells of the fungus , aggregates of membrane systems as well as single cisternae with a diameter of approximately 200 to 300 A can be detected with phase optics. It can be shown, by calculation, that these structures, which are far below the limit of resolution of the light optical system, give enough contrast to be discernible by phase optics. Thus a basis is provided for observing the dynamics of membrane systems which perhaps may contribute to the analysis of the functional significance of these cell components.
通过一种特殊的选择性制备技术，利用电子显微镜在薄切片中研究细胞的某些区域成为可能，这些区域在固定前的活细胞状态下已通过相差显微镜进行了观察。这样就可以将活细胞状态下记录的结构与电子显微镜照片中看到的结构进行比较。在真菌细胞中，利用相差光学显微镜可以检测到膜系统的聚集体以及直径约为200至300埃的单个扁平囊。通过计算可以表明，这些远低于光学系统分辨率极限的结构，能产生足够的对比度以便用相差光学显微镜辨别。因此，为观察膜系统的动态变化提供了基础，这可能有助于分析这些细胞成分的功能意义。
