用于区分柠檬酸杆菌属和沙门氏菌属的β-半乳糖苷酶。
Beta-galactosidase for distinguishing between Citrobacter and Salmonella.
作者信息
Pickett M J, Goodman R E
出版信息
Appl Microbiol. 1966 Mar;14(2):178-82. doi: 10.1128/am.14.2.178-182.1966.
Abstract
Detection of beta-galactosidase with the aid of o-nitrophenyl-beta-d-galactopyranoside (ONPG) was examined as a means for distinguishing between Citrobacter and Salmonella. Several factors which influence sensitivity and reliability of the test were studied. A bacteriostat, sodium azide, was included to permit prolonged incubation of weak and negative strains of enteric bacilli. By the procedure described, salmonellae gave negative ONPG tests; all of 171 strains of Citrobacter gave positive tests.
摘要
借助邻硝基苯-β-D-半乳糖苷（ONPG）检测β-半乳糖苷酶，以此作为区分柠檬酸杆菌属和沙门氏菌属的一种方法进行了研究。对影响该检测灵敏度和可靠性的几个因素进行了研究。加入了一种抑菌剂叠氮化钠，以便对肠道杆菌的弱反应和阴性菌株进行延长培养。按照所述方法，沙门氏菌的ONPG检测呈阴性；171株柠檬酸杆菌属菌株的检测均呈阳性。
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