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[白细胞细胞化学的问题]

[Problems of cytochemistry of leukocytes].

作者信息

Hermanský F, Smetana K, Poch T

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(5):515-23.

PMID:54300
Abstract

In investigating the atypical granula in myeloic cells cytochemically the positivity of DMAB reaction identifies the specific eosinophilic nature of these granula. The slightly modified methods of representing histones allow those histones which are rich of lysine and arginine to be differentiated. With their help those differences can be grasped which exist between the condensed chromatin of lymphocytes and the mature neutrophils caused by the presence of histones being rich of lysine and arginine. New possibilities of examining the proteins in leukocytes in a cytochemical way as well as in blood- and bone-marrow cells are provided by colouring with palatine fast black, the use of which also points to certain differences in the chromatin of lymphocytes and neutrophils.

摘要

在对骨髓细胞中的非典型颗粒进行细胞化学研究时,二甲基氨基联苯胺(DMAB)反应的阳性结果确定了这些颗粒的特异性嗜酸性本质。对组蛋白的表示方法进行了略微修改,使得富含赖氨酸和精氨酸的组蛋白得以区分。借助它们,可以把握淋巴细胞浓缩染色质与成熟中性粒细胞之间因富含赖氨酸和精氨酸的组蛋白的存在而产生的差异。用腭氏固绿染色为以细胞化学方式检查白细胞以及血液和骨髓细胞中的蛋白质提供了新的可能性,其应用也指出了淋巴细胞和中性粒细胞染色质中的某些差异。

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