Edelson E H, Lawless J G, Wehr C T, Abbott S R
J Chromatogr. 1979 Jul 1;174(2):409-19. doi: 10.1016/s0021-9673(00)86015-x.
The high-performance liquid chromatographic separation of a large variety of nucleic acid constituents on a silica-based, weak-anion exchange column was accomplished. Using this technique it was possible to achieve some relatively difficult separations, such as the separation of 2'-, 3'-, and 5'-AMP, and the separation of a mixture of ribo- and deoxyribo-nucleosides and -nucleotides. A number of other separations are demonstrated by isocratic or gradient elution. These include the separation of a mixture of nucleoside monophosphates, the separation of a mixture of nucleoside mono-, di-, and triphosphates, the separation of a mixture of nucleosides and bases, and the separation of a mixture of nucleotide oligomers. These chromatographic separations were accomplished using relatively simple experimental procedures at ambient temperatures and involved relatively short analysis times. Excellent separations were obtained, in most cases, by adjustment of buffer concentration and pH, or by addition of an organic modifier. In some cases, it was necessary to use gradient elution to achieve optimum resolution.
在基于硅胶的弱阴离子交换柱上实现了多种核酸成分的高效液相色谱分离。使用该技术能够实现一些相对困难的分离，例如2'-、3'-和5'-AMP的分离，以及核糖核苷和脱氧核糖核苷与核苷酸混合物的分离。通过等度洗脱或梯度洗脱展示了许多其他分离。这些分离包括核苷单磷酸混合物的分离、核苷单磷酸、二磷酸和三磷酸混合物的分离、核苷和碱基混合物的分离以及核苷酸寡聚物混合物的分离。这些色谱分离在室温下使用相对简单的实验程序完成，且分析时间相对较短。在大多数情况下，通过调整缓冲液浓度和pH值或添加有机改性剂可获得出色的分离效果。在某些情况下，有必要使用梯度洗脱以实现最佳分离度。
