Tsuji K, Goetz J F, vanMeter W, Gusciora K A
J Chromatogr. 1979 Jul 11;175(1):141-52. doi: 10.1016/s0021-9673(00)86409-2.
A high-performance liquid chromatographic (HPLC) assay method has been developed for quantifying neomycin sulfate powders and ointments containing neomycin. A dinitrobenzene derivative of neomycin is formed and then chromatographed isocratically on a normal-phase system. Neomycin B and C and be separated and quantified with a relative standard deviation of approximately 1%. When monitored at 350 nm, the method is sensitive to about 1 ng of neomycin base per column injected. Gradient elution and isocratic HPLC methods for the analysis of derivatized neamine have also been developed. The relative standard deviation is less than 1% for the isocratic method. The HPLC method is applicable to the analysis of other aminoglucoside antibiotics.
已开发出一种高效液相色谱（HPLC）测定方法，用于定量含硫酸新霉素的粉末和软膏。新霉素的二硝基苯衍生物形成后，在正相系统上进行等度色谱分析。新霉素B和C可分离并定量，相对标准偏差约为1%。在350nm处监测时，该方法对每柱注入约1ng新霉素碱敏感。还开发了用于分析衍生新霉素的梯度洗脱和等度HPLC方法。等度法的相对标准偏差小于1%。该HPLC方法适用于其他氨基糖苷类抗生素的分析。
