Butler P J, Jones G M
Biochem J. 1970 Jul;118(3):375-8. doi: 10.1042/bj1180375.
A procedure has been developed for the preparation of alcohol dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase from the same sample of baker's yeast. The two enzymes were obtained in good yield in a pure crystalline form. The method minimizes the work involved in preparing the two enzymes and would be of particular advantage for preparing the enzymes in radioactive form from yeast grown in a radioactive medium.
已开发出一种从同一份面包酵母样品中制备乙醇脱氢酶和3-磷酸甘油醛脱氢酶的方法。这两种酶均以高产量获得纯结晶形式。该方法将制备这两种酶所需的工作量减至最少,对于从在放射性培养基中生长的酵母制备放射性形式的酶而言具有特别的优势。