Bodo M A, Locci P, Cesaroni G, Fronticelli F
Boll Soc Ital Biol Sper. 1979 Nov 15;55(21):2173-9.
Epithelial-mesodermal tissue interactions have been shown to be required for normal cytodifferentiation of chick embryo skin. Six-day limb skin does not develop in a protein free chemically defined medium, but keratinization has been observed in medium containing chicken serum. In the present study the authors show that the addition of human serum may stimulate the in vitro differentiation of explants of six-day chick embryo skin. Human serum is able to support skin keratinization and this finding has been confirmed by histological and histochemical criteria. Synthesis of proteins in tissue cultures supplemented with human serum has been studied by use of labeled amino acids such as H3-Leucine and C14-Cystine. These incorporation studies show the existence of macromolecular factors in human serum which may be responsible for the observed skin differentiation.
上皮-中胚层组织相互作用已被证明是鸡胚皮肤正常细胞分化所必需的。六日龄肢体皮肤在无蛋白化学限定培养基中无法发育，但在含有鸡血清的培养基中已观察到角质化现象。在本研究中，作者表明添加人血清可能会刺激六日龄鸡胚皮肤外植体的体外分化。人血清能够支持皮肤角质化，这一发现已通过组织学和组织化学标准得到证实。通过使用如H3-亮氨酸和C14-胱氨酸等标记氨基酸，研究了添加人血清的组织培养物中蛋白质的合成。这些掺入研究表明人血清中存在大分子因子，它们可能是观察到的皮肤分化的原因。
