Anderson E C, Bell G I
Biophys J. 1971 Mar;11(3):308-21. doi: 10.1016/S0006-3495(71)86217-3.
Error propagation in the Collins-Richmond equation is analyzed in order to obtain the ratio of the fractional error in rate of cell volume increase to the fractional error in each experimental variable. Typical data are analyzed numerically for the total errors resulting from counting statistics, from spectral broadening, and from volume calibration shift. The measurement of 10(4) cells can give a precision of better than 10% in the volume growth rate with a volume resolution of 3%.
分析了柯林斯 - 里士满方程中的误差传播,以获得细胞体积增加速率的分数误差与每个实验变量的分数误差之比。对典型数据进行了数值分析,以计算计数统计、光谱展宽和体积校准偏移所导致的总误差。对10⁴个细胞进行测量,在体积分辨率为3%的情况下,体积增长率的精度可优于10%。