Lozano E A
Can J Comp Med. 1977 Apr;41(2):188-94.
One hundred fifty-one isolates of Clostridium haemolyticum were examined for consistent toxin production following repeated serial transfers in laboratory media. Most of these isolates produced only small amounts of toxic materials and serial transfers appeared to reduce toxigenic characteristics. Eleven of the isolates consistenly produced measurable amounts of toxic materials. One of these isolates was used for production of toxic fluids that were concentrated by lyophilization and reconstitution to a smaller volume or by precipitation with ammonium sulphate followed by dialysis against water and glycerol. Known amounts of these substances were inactivated with formalin, heat, beta-propiolactone, ultra-violet irradiation and glutathione. The resulting toxoids were inoculated into guinea pigs and most were judged to be nonimmunogenic because the animals were unable to resist dermal challenge. Toxic materials with added glycine were inactivated with formaldehyde as readily as those without the amino acid but the resulting toxoids were immunogenic while those prepared without the amino acid were not.
对151株溶血梭菌进行检测，观察其在实验室培养基中反复传代后毒素产生的稳定性。这些菌株大多仅产生少量毒性物质，且传代似乎会降低产毒特性。其中11株能持续产生可测量的毒性物质。选取其中一株用于制备毒性液体，通过冻干浓缩并复溶至较小体积，或用硫酸铵沉淀，随后对水和甘油进行透析。已知量的这些物质用福尔马林、加热、β-丙内酯、紫外线照射和谷胱甘肽进行灭活。将所得类毒素接种到豚鼠体内，大多数被判定为无免疫原性，因为动物无法抵抗皮肤攻击。添加甘氨酸的毒性物质与未添加氨基酸的毒性物质一样容易被甲醛灭活，但所得类毒素具有免疫原性，而未添加氨基酸制备的类毒素则没有。
