Upchurch R G, Elkan G H
Can J Microbiol. 1977 Sep;23(9):1118-22. doi: 10.1139/m77-167.
Four strains of Rhizobium japonicum, two of which produce slimy and non-slimy colony types and two others which produce large and small colony types, were isolated and cloned. All were infective and nodulated Lee soybean host plants. Each colony type was characterized as to its salt sensitivity to Na+ and K+ ions, relative level of symbiotic nitrogen fixation, and relative level of free-living nitrogen fixation. Growth studies performed in the presence of salts demonstrated that the non-slimy or small colony types were sensitive to salt with significantly depressed growth rates and cell yields. Growth rates and cell yields of slimy, large, colony types were relatively unaffected by salt. Both symbiotic and free-living (non-associative) nitrogen fixation analyses (by acetylene reduction) revealed that the non-slimy, small colonies were significantly more effective than slimy, large colonies.
分离并克隆了四株日本根瘤菌，其中两株产生黏液状和非黏液状菌落类型，另外两株产生大菌落和小菌落类型。所有菌株都具有感染性，并能使李氏大豆宿主植物结瘤。对每种菌落类型的耐盐性（对Na+和K+离子）、共生固氮相对水平和自由生活固氮相对水平进行了表征。在有盐存在的情况下进行的生长研究表明，非黏液状或小菌落类型对盐敏感，生长速率和细胞产量显著降低。黏液状、大菌落类型的生长速率和细胞产量相对不受盐的影响。共生和自由生活（非共生）固氮分析（通过乙炔还原）均表明，非黏液状、小菌落比黏液状、大菌落显著更有效。
