Buneaux J J, Buneaux F, Fabiani P
Ann Biol Clin (Paris). 1977;35(2):125-31.
A technic of autoanalysis of the elastase activity and the capacity of elastase inhibition of the serum is described. This estimation depends on the hydrolysis by elastase of N-ter-butoxycarbonyl-L-nitrophenyl ester with liberates p.nitrophenol the colour of which is read at 405 nm. The means of this sample estimation are reported in a few cases of respiratory, dermatological and pancreatic disease.
本文描述了一种血清弹性蛋白酶活性及弹性蛋白酶抑制能力的自动分析技术。该测定取决于弹性蛋白酶对N-叔丁氧羰基-L-硝基苯酯的水解作用,水解后释放出对硝基苯酚,其颜色在405nm处读取。本文报道了该样本测定方法在一些呼吸、皮肤和胰腺疾病病例中的应用情况。