Filfilan S A, Sigee D C
J Cell Sci. 1977;27:81-90. doi: 10.1242/jcs.27.1.81.
The uptake of tritiated thymine into cells of a heterogeneous population of Prorocentrum micans was investigated using light-microscope and electron-microscope autoradiography. Specificity of thymine uptake into DNA was demonstrated by the specific removal of label from wax-embedded material using DNase and by the high degree of localization of nuclear label to chromosomes in the electron-microscope autoradiographs. All nuclei, including both dividing and non-dividing cells, showed a substantial uptake of label, indicating that nuclear DNA synthesis in Prorocentrum micans is a continuous process. The level of DNA synthesis does show considerable variation, however, with very high levels in some interphase nuclei. The continuous replication of nuclear DNA provides further evidence of dinoflagellate affinity to the prokaryotes, and indicates that Prorocentrum micans is a very primitive eukaryote cell.
利用光学显微镜和电子显微镜放射自显影技术，研究了氚标记胸腺嘧啶掺入米氏原甲藻异质群体细胞的情况。通过使用脱氧核糖核酸酶从石蜡包埋材料中特异性去除标记，以及在电子显微镜放射自显影片中核标记高度定位于染色体，证明了胸腺嘧啶掺入DNA的特异性。所有细胞核，包括正在分裂和未分裂的细胞，都显示出大量的标记摄取，这表明米氏原甲藻中的核DNA合成是一个连续的过程。然而，DNA合成水平确实存在相当大的差异，一些间期核中的水平非常高。核DNA的连续复制进一步证明了甲藻与原核生物的亲缘关系，并表明米氏原甲藻是一种非常原始的真核细胞。
