不同形式的DNA在固定化组蛋白柱上的色谱分析。

Chromatography of different forms of DNA on immobilized histone columns.

作者信息

Yu S H, Lau P P, Spring T G

出版信息

Biochim Biophys Acta. 1978 Jan 26;517(1):31-42. doi: 10.1016/0005-2787(78)90031-x.

Abstract

Histones 1, 2A, 2B, 3, 4, and 5 from chicken erythrocytes have each been covalently immobilized on agarose beads and tested for their ability to bind DNA. Both calf thymus DNA and PM-2 viral DNA (form II, a nicked circle) bind to columns of immobilized histone at low ionic strength and can be eluted with 0.2--0.8 M NaC1, depending on the histone column. The strength of DNA binding by histone columns is in the order H5 greater than H1 greater than greater than H2B approximately H3 greater than H4 approximately H2A. About 70% of a heat-denatured calf thymus DNA sample and 100% of a supercoiled (form I) PM-2 DNA sample bind very tightly to histone columns and are released only by strong protein denaturants.

摘要

来自鸡红细胞的组蛋白1、2A、2B、3、4和5已分别共价固定在琼脂糖珠上，并测试了它们结合DNA的能力。小牛胸腺DNA和PM - 2病毒DNA（形式II，带切口的环状）在低离子强度下都能与固定化组蛋白柱结合，并可根据组蛋白柱用0.2 - 0.8M NaCl洗脱。组蛋白柱结合DNA的强度顺序为H5大于H1大于大于H2B约等于H3大于H4约等于H2A。约70%的热变性小牛胸腺DNA样品和100%的超螺旋（形式I）PM - 2 DNA样品与组蛋白柱紧密结合，只有通过强蛋白质变性剂才能释放。