硫脲可逆转经二氯二氨铂（II）处理的DNA中的交联，并恢复其生物活性。
Thiourea reverses cross-links and restores biological activity in DNA treated with dichlorodiaminoplatinum (II).
作者信息
Filipski J, Kohn K W, Prather R, Bonner W M
出版信息
Science. 1979 Apr 13;204(4389):181-3. doi: 10.1126/science.571145.
PMID:571145
Abstract
Cis and trans dichlorodiaminoplatinum (II) compounds bind to DNA and form DNA cross-links, which are usually considered to be irreversible. Thiourea can reverse these cross-links without any apparent breakdown of the DNA. In addition, cis- and trans-Pt (II) treatment of lambda decreases its transfectivity. After suitable incubation with thiourea, full transfectivity of Pt(II)-treated lambda DNA can be restored.
摘要
顺式和反式二氯二氨铂（II）化合物与DNA结合并形成DNA交联，通常认为这种交联是不可逆的。硫脲可以逆转这些交联，而DNA没有任何明显的断裂。此外，用顺式和反式Pt（II）处理λ噬菌体可降低其转染能力。在用硫脲进行适当孵育后，经Pt（II）处理的λ噬菌体DNA的转染能力可完全恢复。
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