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多聚甲醛诱导荧光法作为负鼠附睾中5-羟色胺的组织化学检测方法。

Paraformaldehyde-induced fluorescence as a histochemical test for 5-hydroxytryptamine in the epididymis of the opossum.

作者信息

Anderson M E, Paparo A A, Martan J

出版信息

J Anat. 1979 Aug;129(Pt 1):141-9.

PMID:574505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1233089/
Abstract

Epididymides of four opossums were tested histochemically for the presence of 5-hydroxytryptamine (5-HT). In addition, testes and epididymides of two opossums were analysed biochemically for the same substance. Biochemical analysis revealed a high concentration of 5-HT in the epididymis, while the amount in the testis proved to be insignificant. Fluorescence microscopy gave positive results in the apical portion of the epithelium. There was also fluorescence in the connective tissue in general, in mast cells, and in a thick subepithelial band in particular. Following treatment with 2% sodium borohydride in 1,2-propanediol for 15 minutes, the fluorescence of mast cells was removed, while there was a slight reduction in epithelial fluorescence and that in the subepithelial band and other connective tissue. The subepithelial band stained metachromatically with azure B, and this staining characteristic was removed by treatment with RNAse. Feulgen reaction revealed elongate nuclei in the band. It would seem that the 5-HT is located in both the mast cells and the epithelium. It seemed to be also present in the subepithelial band, but because of strong autofluorescence it is difficult to demonstrate. At the present time the function of 5-HT in the opossum epididymis is unknown, and is under further investigation.

摘要

对四只负鼠的附睾进行了组织化学检测，以确定是否存在5-羟色胺（5-HT）。此外，对两只负鼠的睾丸和附睾进行了该物质的生化分析。生化分析显示附睾中5-HT浓度很高，而睾丸中的含量则微不足道。荧光显微镜检查在上皮细胞顶端部分得到阳性结果。一般结缔组织、肥大细胞以及特别是上皮下厚带中也有荧光。用2%硼氢化钠的1,2-丙二醇溶液处理15分钟后，肥大细胞的荧光消失，而上皮荧光以及上皮下带和其他结缔组织中的荧光略有减弱。上皮下带用天青B进行异染性染色，这种染色特性经核糖核酸酶处理后消失。福尔根反应显示该带中有细长的细胞核。5-HT似乎既存在于肥大细胞中，也存在于上皮细胞中。它似乎也存在于上皮下带，但由于强烈的自发荧光，很难证实。目前，5-HT在负鼠附睾中的功能尚不清楚，正在进一步研究中。

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本文引用的文献

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FLUORESCENCE METHODS FOR THE HISTOCHEMICAL DEMONSTRATION OF MONOAMINES. 3. SODIUM BOROHYDRIDE REDUCTION OF THE FLUORESCENT COMPOUNDS AS A SPECIFICITY TEST.用于单胺组织化学显示的荧光方法。3. 硼氢化钠对荧光化合物的还原作为特异性测试。
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Isolation and purification of acid phosphatase-containing autofluorescent granules from homogenates of rat kidney cortex.从大鼠肾皮质匀浆中分离和纯化含酸性磷酸酶的自发荧光颗粒。
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