组织胞浆菌病研究IV. 组织抑制因子（HIF）的洗脱特性
Studies on histoplasmosis IV. Elution characteristics of the histo-inhibitory factor (HIF).
作者信息
O'Hern E M, Fishbein L
出版信息
Mycopathol Mycol Appl. 1968 Oct 31;36(1):33-41. doi: 10.1007/BF02057164.
Abstract
The histo-inhibitory factor (HIF) derived from homogenates of liver or kidney from hamsters infected with has been fractionated by column chromatography. It shows maximum absorption at 280 m, has a molecular weight of 142,000 and can be eluted from DEAE-cellulose or DEAE-Sephadex A—50 with 0.02 M phosphate — 4 M sodium chloride (1 : 1) HIF can be eluted from yeast cells at pH 10.0, thus a greater number of positive cultures from chronic histoplasmosis could be expected to result from pretreatment of clinical specimens in glycine buffer pH 10.0 prior to culture.
摘要
从感染了的仓鼠肝脏或肾脏匀浆中提取的组织抑制因子（HIF）已通过柱色谱法进行了分级分离。它在280 m处有最大吸收峰，分子量为142,000，可用0.02 M磷酸盐 - 4 M氯化钠（1:1）从DEAE - 纤维素或DEAE - 葡聚糖A - 50上洗脱下来。HIF在pH 10.0时可从酵母细胞中洗脱下来，因此预计在培养前将临床标本在pH 10.0的甘氨酸缓冲液中预处理，慢性组织胞浆菌病会产生更多阳性培养物。
相似文献
1
Studies on histoplasmosis IV. Elution characteristics of the histo-inhibitory factor (HIF).组织胞浆菌病研究IV. 组织抑制因子（HIF）的洗脱特性
Mycopathol Mycol Appl. 1968 Oct 31;36(1):33-41. doi: 10.1007/BF02057164.
2
Studies in histoplasmosis. 3. Cross reactions and characterization of Histoplasma and Blastomyces inhibitory factors.组织胞浆菌病研究。3. 组织胞浆菌和芽生菌抑制因子的交叉反应及特性
Mycopathol Mycol Appl. 1967 Apr 28;31(3):193-208. doi: 10.1007/BF02053417.
3
Factor in acidified serum toxic for malignant cells in culture.培养中对恶性细胞有毒性的酸化血清中的因素。
Nat New Biol. 1973 Apr 11;242(119):186-8. doi: 10.1038/newbio242186a0.
4
Antigens of Histoplasma capsulatum. II. Separation and characterization of yeast-phase precipitinogens.荚膜组织胞浆菌抗原。II. 酵母相沉淀原的分离与特性鉴定
Am Rev Respir Dis. 1971 Sep;104(3):401-7. doi: 10.1164/arrd.1971.104.3.401.
5
STUDIES ON HISTOPLASMOSIS. II. THE HISTOPLASMA INHIBITORY FACTOR IN HAMSTERS INOCULATED INTRADERMALLY OF SUBCUTANEOUSLY.
J Immunol. 1965 Apr;94:592-602.
6
Factors governing the epidemiology of Histoplasma capsulatum in soil.
Mycopathol Mycol Appl. 1970;41(1):167-76. doi: 10.1007/BF02051492.
7
Studies on histoplasmosis. V. Protective effect of HIF-coated Histoplasma against challenge.组织胞浆菌病研究。V. 包被有透明质酸铁的荚膜组织胞浆菌对攻击的保护作用。
Mycopathol Mycol Appl. 1969 Jan 29;37(1):57-64. doi: 10.1007/BF02051331.
8
[HISTOPLASMA CAPSULATUM IN SOIL. II. MORPHOLOGICAL ASPECTS OF THE FUNGUS, TISSUE REACTIONS AND COMPARISON OF CULTURE EXPERIMENTS WITH HISTO- PLASMA CAPSULATUM AND HISTOLOGICAL INVESTIGATIONS IN MICE].土壤中的荚膜组织胞浆菌。II. 真菌的形态学特征、组织反应以及荚膜组织胞浆菌培养实验与小鼠组织学研究的比较
Mycopathol Mycol Appl. 1963 May 20;19:62-82. doi: 10.1007/BF02049015.
9
STUDIES ON HISTOPLASMOSIS. I. COMPARATIVE VIRULENCE OF VARIANT AND PARENT STRAIN HISTOPLASMA CAPSULATUM IN HAMSTERS.组织胞浆菌病研究。I. 荚膜组织胞浆菌变异株和亲本株在仓鼠体内的比较毒力
Mycopathol Mycol Appl. 1964 Apr 30;22:167-74. doi: 10.1007/BF02049651.
10
THE INFLUENCE OF PROPYLENE GLYCOL ON THE GROWTH OF HISTOPLASMA CAPSULATUM IN VITRO AND IN EXPERIMENTAL INFECTION.
Mycopathol Mycol Appl. 1964 Apr 30;22:225-7. doi: 10.1007/BF02049658.
引用本文的文献
1
Studies on histoplasmosis. V. Protective effect of HIF-coated Histoplasma against challenge.组织胞浆菌病研究。V. 包被有透明质酸铁的荚膜组织胞浆菌对攻击的保护作用。
Mycopathol Mycol Appl. 1969 Jan 29;37(1):57-64. doi: 10.1007/BF02051331.
本文引用的文献
1
Resistance of hamsters in infections with Histoplasma capsulatum.
J Immunol. 1961 Dec;87:728-36.
2
STUDIES ON HISTOPLASMOSIS. II. THE HISTOPLASMA INHIBITORY FACTOR IN HAMSTERS INOCULATED INTRADERMALLY OF SUBCUTANEOUSLY.
J Immunol. 1965 Apr;94:592-602.
3
THE EPIDERMAL CHALONE; A PRELIMINARY ATTEMPT AT ISOLATION.表皮抑素；分离的初步尝试
Exp Cell Res. 1964 Oct;36:192-200. doi: 10.1016/0014-4827(64)90172-7.
4
STUDIES ON THE CHROMATOGRAPHY OF HUMAN SERUM PROTEINS ON DIETHYLAMINOETHYL(DEAE)-CELLULOSE. I. THE EFFECT OF THE CHEMICAL AND PHYSICAL NATURE OF THE EXCHANGER.人血清蛋白在二乙氨基乙基（DEAE）-纤维素上的色谱研究。I. 交换剂化学和物理性质的影响
J Chromatogr. 1964 Aug;15:324-35. doi: 10.1016/s0021-9673(01)82790-4.
5
Estimation of molecular weights of proteins by gel filtration.通过凝胶过滤法估算蛋白质的分子量
Nature. 1962 Oct 6;196:36-9. doi: 10.1038/196036a0.
6
7
Protein chromatography on ion exchange cellulose.离子交换纤维素上的蛋白质色谱法。
Fed Proc. 1958 Dec;17(4):1116-26.
8
Humoral defense mechanisms in cryptococcosis: substances in normal human serum, saliva, and cerebrospinal fluid affecting the growth of Cryptococcus neoformans.隐球菌病中的体液防御机制：正常人血清、唾液和脑脊液中影响新型隐球菌生长的物质。
J Infect Dis. 1966 Feb;116(1):75-83. doi: 10.1093/infdis/116.1.75.
9
Inhibition of growth of malignant cells in vitro by a component of normal adult connective tissue.正常成人结缔组织的一种成分对体外恶性细胞生长的抑制作用。
Nature. 1965 Nov 20;208(5012):800-1. doi: 10.1038/208800a0.
10
The chemistry of xanthine oxidase. 11. Ultracentrifuge and gel-filtration studies on the milk enzyme.黄嘌呤氧化酶的化学性质。11. 对乳中该酶的超速离心和凝胶过滤研究。
Biochem J. 1964 Dec;93(3):627-32. doi: 10.1042/bj0930627.
Zotero 插件