Simpson L L, Morimoto H
J Bacteriol. 1969 Feb;97(2):571-5. doi: 10.1128/jb.97.2.571-575.1969.
An attempt has been made to replicate an earlier finding that type A botulinum toxin can inhibit the in vitro activity of acetylcholinesterase. Two methods of enzyme assay were employed, but with neither technique were we able to reproduce the finding of in vitro enzyme inhibition. In fact, an examination of the data from the previous report leads us to question the possibility of the observations that were given. Furthermore, an investigation was carried out to determine if botulinum toxin can exert an inhibiting effect on acetylcholinesterase that is situated in the biological tissue. The answer again is negative. The experimental observations, coupled with several mathematical computations, do not support the notion that botulinum toxin is an acetylcholinesterase inhibitor.
人们曾试图复制早期的一项发现，即A型肉毒杆菌毒素可抑制乙酰胆碱酯酶的体外活性。采用了两种酶测定方法，但使用这两种技术我们都未能重现体外酶抑制的发现。事实上，对先前报告中的数据进行检查后，我们对所给出观察结果的可能性产生了质疑。此外，还进行了一项调查，以确定肉毒杆菌毒素是否能对生物组织中的乙酰胆碱酯酶产生抑制作用。答案同样是否定的。实验观察结果以及几项数学计算都不支持肉毒杆菌毒素是一种乙酰胆碱酯酶抑制剂这一观点。
