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耐氧型小球藻(索氏小球藻)菌株

Oxygen-tolerant strain of chlorella sorokiniana.

作者信息

Wagner F W, Welch B E

出版信息

Appl Microbiol. 1969 Jan;17(1):139-44. doi: 10.1128/am.17.1.139-144.1969.

Abstract

An oxygen-tolerant strain (OTS) of Chlorella sorokiniana was isolated by growing and continuously subculturing the oxygen-sensitive strain (OSS) in growth medium bubbled continuously with 95% O(2)-5% CO(2). Under these conditions, 6 to 8 hr were required before the OSS began to grow. The growth rates of the OTS and the OSS were the same when grown in an atmosphere of air-5% CO(2), and the growth rate of the OTS was the same when aerated with 95% O(2)-5% CO(2) and air-5% CO(2). The adaptive process was irreversible since serial transfers of the OTS grown with air-5% CO(2) did not alter the ability of the OTS to grow with 95% O(2)-5% CO(2). Inasmuch as photosynthesis in the OTS and the OSS was inhibited to a similar extent by oxygen and the adaptive process occurred heterotrophically in the absence of photosynthesis, it was concluded that the adaptive process was independent of photosynthesis. Morphological differences between the OTS and the OSS are presented and discussed with respect to the adaptive process.

摘要

通过在持续通入95% O₂ - 5% CO₂ 的生长培养基中培养并连续传代氧气敏感型小球藻菌株(OSS),分离出了一株耐氧型小球藻菌株(OTS)。在这些条件下,OSS开始生长前需要6至8小时。OTS和OSS在空气 - 5% CO₂ 气氛中生长时生长速率相同,并且OTS在通入95% O₂ - 5% CO₂ 和空气 - 5% CO₂ 时生长速率相同。由于用空气 - 5% CO₂ 培养的OTS连续传代并没有改变其在95% O₂ - 5% CO₂ 中生长的能力,所以适应性过程是不可逆的。鉴于OTS和OSS中的光合作用受到氧气抑制的程度相似,并且适应性过程在没有光合作用的情况下以异养方式发生,因此得出结论,适应性过程与光合作用无关。文中呈现并讨论了OTS和OSS之间在适应性过程方面的形态差异。

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本文引用的文献

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Oxygen as a factor in photosynthesis.氧气作为光合作用中的一个因素。
Biol Rev Camb Philos Soc. 1962 Feb;37:130-70. doi: 10.1111/j.1469-185x.1962.tb01607.x.

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