借助负染色-碳膜技术制备的烟草花叶病毒的电子显微镜观察。

Electron microscopy of tobacco mosaic virus prepared with the aid of negative staining-carbon film techniques.

作者信息

Horne R W, Hobart J M, Markham R

出版信息

J Gen Virol. 1976 May;31(2):265-9. doi: 10.1099/0022-1317-31-2-265.

Abstract

The negative staining-carbon technique has been applied to suspensions of freshly prepared type strain tobacco mosaic virus particles in high concentrations. Electron microscope images show that paracrystalline arrays of the virus were formed, in which large areas of rods could be viewed along their long axes and in parallel arrays in horizontal positions. High-resolution micrographs showed the protein structure units in rods photographed in both vertical and horizontal orientations.

摘要

负染色-碳技术已应用于高浓度新鲜制备的烟草花叶病毒标准株颗粒悬液。电子显微镜图像显示形成了病毒的准晶体阵列，其中大片的杆状病毒粒子可沿其长轴并在水平位置以平行阵列形式观察到。高分辨率显微照片显示了在垂直和水平方向拍摄的杆状病毒粒子中的蛋白质结构单元。