Butler M S, Fried B
J Parasitol. 1977 Dec;63(6):1041-5.
Excysted metacercariae of Echinostoma revolutum cultured in vitro in the defined medium, NCTC 135 supplemented with 20% hens' egg yolk, doubled their mean relative body area and showed significant sucker growth within 14 days. Histochemical Oil Red O staining showed neutral fat mainly in the excretory system of excysted metacercariae and in adults grown in the domestic chick. In vitro cultured worms showed neutral fat in the intestine, parenchyma, and excretory system. As detected by TLC the major neutral lipid fractions were free fatty acids for excysted metacercariae; free sterols for adults grown in chicks; and triglycerides, free fatty acids, and free sterols for cultured worms. Excysted metacercariae excreted free fatty acids into a nonnutrient incubation medium, whereas cultured worms excreted diglycerides, triglycerides, and free fatty acids into the medium.
在添加20%鸡蛋黄的限定培养基NCTC 135中体外培养的卷棘口吸虫脱囊后尾蚴，其平均相对体面积在14天内增加了一倍，并出现明显的吸盘生长。组织化学油红O染色显示，中性脂肪主要存在于脱囊后尾蚴的排泄系统以及在家鸡体内生长的成虫中。体外培养的虫体在肠道、实质组织和排泄系统中显示有中性脂肪。通过薄层色谱法检测，脱囊后尾蚴的主要中性脂质成分是游离脂肪酸；在鸡体内生长的成虫是游离固醇；而培养的虫体是甘油三酯、游离脂肪酸和游离固醇。脱囊后尾蚴将游离脂肪酸排泄到无营养的孵育培养基中，而培养的虫体则将甘油二酯、甘油三酯和游离脂肪酸排泄到培养基中。
