Siedlecki J A, Mikke R, Zmudzka B
Acta Biochim Pol. 1976;23(1):69-84.
In the post-mitochondrial fraction of murine LBN/b leukemic cells, four fractions with DNA polymerase activity (I, II, III, IV) were found. On the basis of ion exchanger affinity and poly(A), poly(C) and poly(Cm) replication ability, fraction I was classified as RNA-directed DNA polymerase of viral origin. On the basis of the differences in the ion exchanger affinity, molecular weight, template requirement, pH-dependence of enzymatic activity and NaCl concentration, divalent ion requirements and susceptibility to N-ethylmaleimide inhibition, fractions II, III and IV were classified as DNA-directed DNA polymerases beta, alpha and gamma, respectively. Three fractions, i.e. reverse transcriptase, and DNA-directed DNA polymerases beta and gamma, were found to incorporate dTMP on a poly(A)-oligo(dT) template-primer. Despite the similarity of the reaction of DNA polymerases beta and gamma with poly(A)-oligo(dT), some other properties of these enzymes suggest that they represent distinct enzymatic entities.
在小鼠LBN/b白血病细胞的线粒体后组分中，发现了具有DNA聚合酶活性的四个组分（I、II、III、IV）。根据离子交换剂亲和力以及对聚（A）、聚（C）和聚（Cｍ）的复制能力，组分I被归类为病毒来源的RNA指导的DNA聚合酶。根据离子交换剂亲和力、分子量、模板需求、酶活性的pH依赖性和NaCl浓度、二价离子需求以及对N-乙基马来酰亚胺抑制的敏感性等方面的差异，组分II、III和IV分别被归类为DNA指导的DNA聚合酶β、α和γ。发现三个组分，即逆转录酶以及DNA指导的DNA聚合酶β和γ，能够在聚（A）-寡聚（dT）模板引物上掺入dTMP。尽管DNA聚合酶β和γ与聚（A）-寡聚（dT）的反应相似，但这些酶的其他一些特性表明它们代表不同的酶实体。
