Schapendonk A H, Vredenberg W J
Biochim Biophys Acta. 1977 Dec 23;462(3):613-21. doi: 10.1016/0005-2728(77)90105-0.
Absorbance changes, caused by adding KCl to a suspension of broken chloroplasts in the presence of a low concentration of MgCl2, have been measured in the wavelength region 460-540 nm. The magnitude of the KCl-induced absorbance changes is shown to be proportional to the logarithm of the KCL concentration gradient initially induced across the thylakoid membrane. The difference spectrum of these absorbance changes is shown to be identical with the spectrum of the light-induced absorbance changes, which has been attributed to an electrochromic shift of p-515. This is interpreted as evidence that under these conditions salt-induced absorbance changes of P-515 occur in response to a membrane diffusion potential. The results indicate that the electrogenic potential across the thylakoid membrane, generated by a single turnover light flash, is in the range between 15 and 35 mV.
在低浓度氯化镁存在的情况下，向破碎叶绿体的悬浮液中添加氯化钾所引起的吸光度变化，已在460 - 540纳米波长范围内进行了测量。结果表明，氯化钾诱导的吸光度变化幅度与最初在类囊体膜上诱导的氯化钾浓度梯度的对数成正比。这些吸光度变化的差光谱与光诱导吸光度变化的光谱相同，后者被归因于p - 515的电致变色位移。这被解释为在这些条件下，P - 515的盐诱导吸光度变化是对膜扩散电位的响应的证据。结果表明，由单次周转光闪光产生的跨类囊体膜的电生电位在15至35毫伏之间。
