利用¹⁴C-亮氨酸掺入法研究植物血凝素诱导的正常淋巴细胞对组织培养细胞的细胞毒性作用。

Phytohemagglutinin-induced cytotoxic action of normal lymphocytes on cells in tissue culture using 14C-leucine incorporation.

作者信息

Nemoto R, Ishikawa K, Kato T

出版信息

Tohoku J Exp Med. 1977 Dec;123(4):389-91. doi: 10.1620/tjem.123.389.

DOI:10.1620/tjem.123.389

Abstract

Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transformation stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorporation in to target cells after interaction with PHA-treated PBL. The cytotoxicity of PHA-treated PBL showed dose response curves which corresponded with that of PBL transformation. Reduced 14C-leucine incorporation of target cells caused by cytotoxic PBL would be a reliable measure of immune reactions.

摘要

研究了植物血凝素（PHA）对正常外周血淋巴细胞（PBL）的刺激作用以及PHA诱导的细胞毒性的产生。通过3H-胸腺嘧啶核苷掺入法测定PHA刺激的PBL转化，通过测量与PHA处理的PBL相互作用后靶细胞中14C-亮氨酸掺入的减少来测定细胞毒性。PHA处理的PBL的细胞毒性显示出与PBL转化相对应的剂量反应曲线。由细胞毒性PBL引起的靶细胞14C-亮氨酸掺入减少将是免疫反应的可靠指标。

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