哺乳动物基因组研究中的羟基磷灰石分级分离程序。
Hydroxyapatite fractionation procedures in the study of the mammalian genome.
作者信息
McCallum M, Walker P M
出版信息
Biochem J. 1967 Oct;105(1):163-9. doi: 10.1042/bj1050163.
Abstract
Three methods of fractionating DNA are described, in which DNA fragments are separated on the basis (1) of their base composition, (2) of the ability of denatured DNA to renature after incubation, and (3) of the presence of intrastrand secondary structure in denatured DNA. The ability of some of these fractions to form specific duplexes with whole denatured DNA isolated from the same and from a different mammal has been investigated. The results are presented to demonstrate how the fractionation procedures described may provide new information about the organization of the mammalian genome.
摘要
本文描述了三种分离DNA的方法，其中DNA片段是基于以下因素进行分离的：（1）碱基组成；（2）变性DNA在孵育后复性的能力；（3）变性DNA中链内二级结构的存在。研究了其中一些组分与从同一哺乳动物和不同哺乳动物分离的全变性DNA形成特异性双链体的能力。展示这些结果是为了说明所描述的分离程序如何能够提供有关哺乳动物基因组组织的新信息。
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