特定胆碱池在大鼠心脏鞘磷脂合成中的作用
Role of a specific choline pool in sphingomyelin synthesis in rat heart.
作者信息
Kiss Z
出版信息
Biochem J. 1977 Dec 15;168(3):387-91. doi: 10.1042/bj1680387.
Sphingomyelin synthesis was studied in slices of rat heart by using [Me-14C]choline, [1,2-14C]ethanolamine, S-adenosyl-L-[14C]methionine and [32P]Pi as as precursors. In the presence of both [Me-14C]choline and [32P]Pi the ratio of the specific radioactivities of 14C and 32P in phosphatidylcholine was greater than in sphingomyelin at all the times studied. This suggested that synthesis of phosphatidylcholine and sphingomyelin de novo did not involve the utilization of a common pool of cytidine diphosphate choline. In addition, studies with [1,2-14C]ethanolamine and S-adenosyl-L-[14C]methionine indicated that a quantitatively significant pool of choline, derived from these precursors, was selectively utilized for sphingomyelin formation. This pool was not represented by phosphatidylcholine formed by methylation of phosphatidylethanolamine or by other pathways.
利用[甲基-14C]胆碱、[1,2-14C]乙醇胺、S-腺苷-L-[14C]甲硫氨酸和[32P]无机磷酸作为前体，对大鼠心脏切片中的鞘磷脂合成进行了研究。在同时存在[甲基-14C]胆碱和[32P]无机磷酸的情况下，在所研究的所有时间点，磷脂酰胆碱中14C和32P的比放射性比值均大于鞘磷脂中的该比值。这表明磷脂酰胆碱和鞘磷脂的从头合成并不涉及利用共同的胞苷二磷酸胆碱池。此外，用[1,2-14C]乙醇胺和S-腺苷-L-[14C]甲硫氨酸进行的研究表明，源自这些前体的数量可观的胆碱池被选择性地用于鞘磷脂的形成。该池并非由磷脂酰乙醇胺甲基化形成的磷脂酰胆碱或其他途径形成的磷脂酰胆碱所代表。
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