Ray P M
J Cell Biol. 1967 Dec;35(3):659-74. doi: 10.1083/jcb.35.3.659.
Segments cut from growing oat coleoptiles and pea stems were fed glucose-(3)H in presence and absence of the growth hormone indoleacetic acid (IAA). By means of electron microscope radioautography it was demonstrated that new cell wall material is deposited both at the wall surface (apposition) and within the preexisting wall structure (internally). Quantitative profiles for the distribution of incorporation with position through the thickness of the wall were obtained for the thick outer wall of epidermal cells. With both oat coleoptile and pea stem epidermal outer walls, it was found that a larger proportion of the newly synthesized wall material appeared to become incorporated within the wall in the presence of IAA. Extraction experiments on coleoptile tissue showed that activity that had been incorporated into the cell wall interior represented noncellulosic constituents, mainly hemicelluloses, whereas cellulose was deposited largely or entirely by apposition. It seems possible that internal incorporation of hemicelluloses plays a role in the cell wall expansion process that is involved in cell growth.
从生长中的燕麦胚芽鞘和豌豆茎上切下的切段，在有和没有生长激素吲哚乙酸（IAA）的情况下用葡萄糖 -（3）H 进行喂养。通过电子显微镜放射自显影表明，新的细胞壁物质既沉积在细胞壁表面（附着），也沉积在先前存在的细胞壁结构内部（内部）。对于表皮细胞的厚外壁，获得了沿着细胞壁厚度方向掺入量分布的定量图谱。对于燕麦胚芽鞘和豌豆茎的表皮外壁，发现在有 IAA 的情况下，新合成的细胞壁物质中较大比例似乎掺入到细胞壁内部。对胚芽鞘组织的提取实验表明，掺入到细胞壁内部的活性代表非纤维素成分，主要是半纤维素，而纤维素主要或完全通过附着沉积。半纤维素的内部掺入似乎可能在涉及细胞生长的细胞壁扩展过程中起作用。
