Epstein N
Dev Biol Stand. 1984;57:129-32.
The aim of this article is to review the role of monoclonal antibodies as a tool in malaria research. In particular we concentrate on a monoclonal antibody directed against a functional epitope on the merozoite surface. This antibody blocked the invasion of merozoites into the red blood cells by causing agglutination of the parasite. The antigenic determinant recognized by this mcAb, 13C11, is located on a major 230 K dalton glycoprotein of the mature Plasmodium knowlesi parasite. Using the 13C11 monoclonal antibody it was possible to follow the synthesis and insertion of this glycoprotein into the schizont membrane. The relation between the intraerythrocyte schizont and the extraerythrocyte merozoite components recognized by the 13C11 were defined. Furthermore, 13C11 was used to analyze the processing of the 230 Kd plasma-membrane component to lower molecular weight products which were present on the surface of the invasive merozoite and which might play a role in the invasion process.
本文旨在综述单克隆抗体作为疟疾研究工具的作用。特别地，我们聚焦于一种针对裂殖子表面功能性表位的单克隆抗体。该抗体通过引起寄生虫凝集，阻断了裂殖子侵入红细胞。这种单克隆抗体（13C11）识别的抗原决定簇位于成熟诺氏疟原虫主要的230千道尔顿糖蛋白上。利用13C11单克隆抗体，可以追踪这种糖蛋白在裂殖体膜中的合成与插入。确定了13C11识别的红细胞内裂殖体与红细胞外裂殖子成分之间的关系。此外，13C11被用于分析230千道尔顿质膜成分向低分子量产物的加工过程，这些低分子量产物存在于侵袭性裂殖子表面，可能在入侵过程中发挥作用。
