Johnson B F
Arch Microbiol. 1984 Jun;138(2):106-12. doi: 10.1007/BF00413009.
A previously undescribed mutation in the ssb gene, which codes for a major single strand DNA binding protein essential for DNA replication, was mapped on the Escherichia coli chromosome. Three ssb mutants were analyzed under parallel physiological conditions for the induction of SOS proteins (products of recA, uvrA, and an unknown gene), the production of mutants, the induction of lambda prophage, and sensitivity to DNA damaging agents. Two-dimensional electrophoretic techniques were used to quantitate changes in the rate of synthesis of proteins. The previously unpublished position of the uvrA gene-product in the two-dimensional matrix of E. coli proteins was described. These ssb strains exhibited varying sensitivities to ultraviolet irradiation and methylmethane sulfonate that correlated with the rate of constitutive synthesis of SOS proteins, spontaneous commitment to virulent growth of lambda lysogens, and elevation of endogenous mutation rates.
编码DNA复制所必需的一种主要单链DNA结合蛋白的ssb基因中，一种先前未被描述的突变被定位到了大肠杆菌染色体上。在平行生理条件下，对三个ssb突变体进行了分析，以研究SOS蛋白（recA、uvrA和一个未知基因的产物）的诱导、突变体的产生、λ原噬菌体的诱导以及对DNA损伤剂的敏感性。使用二维电泳技术来定量蛋白质合成速率的变化。描述了uvrA基因产物在大肠杆菌蛋白质二维矩阵中先前未发表的位置。这些ssb菌株对紫外线照射和甲基磺酸甲酯表现出不同的敏感性，这与SOS蛋白的组成型合成速率、λ溶原菌向毒性生长的自发转变以及内源性突变率的升高相关。
