Rainteau D, Wolf C, Bereziat G, Polonovski J
Biochem J. 1984 Aug 1;221(3):659-63. doi: 10.1042/bj2210659.
The binding of a spin-labelled derivative of chlorpromazine to calmodulin was investigated by e.s.r. spectrometry. The completion of the spectroscopic changes requires the presence of 4 Ca2+ ions per calmodulin molecule. The influences of various physicochemical factors (pH, ionic strength) are discussed in relation to the nature (hydrophobic and polar) of the interactions that hold the drug-calmodulin complex together.
通过电子自旋共振光谱法研究了氯丙嗪的自旋标记衍生物与钙调蛋白的结合。光谱变化的完成需要每个钙调蛋白分子存在4个Ca2+离子。讨论了各种物理化学因素(pH值、离子强度)对维系药物 - 钙调蛋白复合物的相互作用性质(疏水和极性)的影响。