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人载脂蛋白A-II:克隆cDNA的核苷酸序列及其结构基因在人染色体1上的定位。

Human apolipoprotein A-II: nucleotide sequence of a cloned cDNA, and localization of its structural gene on human chromosome 1.

作者信息

Moore M N, Kao F T, Tsao Y K, Chan L

出版信息

Biochem Biophys Res Commun. 1984 Aug 30;123(1):1-7. doi: 10.1016/0006-291x(84)90371-1.

Abstract

Using the technique of oligonucleotide hybridization, we have isolated two dscDNA clones to human apolipoprotein A-II. One of the clones (pAII-1) has been completely sequenced. It has 433 nucleotides which includes a poly A tail of 10 adenylic acid residues, all the coding and 3'-non-translated regions of the mRNA and part of the 5'-non-translated region. The amino acid sequence derived from the cDNA clone includes 100 amino acids (including the 23 amino acid prepropeptide) which is very similar to the sequence reported by Brewer et al. (Proc. Natl. Acad. Sci. USA 69, 1304-1308). [32P]-labeled pAII-1 was used as a probe in chromosome mapping studies to detect the human apoAII structural gene sequence in human-Chinese hamster cell hybrids. Southern blot analysis of 10 hybrids localized the gene to human chromosome 1.

摘要

利用寡核苷酸杂交技术,我们分离出了两个人载脂蛋白A-II的双链互补DNA克隆。其中一个克隆(pAII-1)已被完全测序。它有433个核苷酸,包括一个由10个腺苷酸残基组成的聚腺苷酸尾、mRNA的所有编码区和3'非翻译区以及部分5'非翻译区。从该cDNA克隆推导的氨基酸序列包括100个氨基酸(包括23个氨基酸的前原肽),这与布鲁尔等人报道的序列非常相似(《美国国家科学院院刊》69,1304 - 1308)。用[32P]标记的pAII-1作为探针进行染色体定位研究,以检测人 - 中国仓鼠细胞杂种中的人载脂蛋白AII结构基因序列。对10个杂种的Southern印迹分析将该基因定位到人类第1号染色体上。

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