[125I] -血管紧张素II受体在大鼠肾皮质上皮细胞上的定位。
Location of [125I]-angiotensin II receptors on rat kidney cortex epithelial cells.
作者信息
Cox H M, Munday K A, Poat J A
出版信息
Br J Pharmacol. 1984 Aug;82(4):891-5. doi: 10.1111/j.1476-5381.1984.tb16487.x.
Abstract
Angiotensin II (AII) stimulates active Na+ extrusion from Na+ loaded renal cortex slices. Specific high affinity [125I]-AII binding sites in partially purified basolateral and brush-border epithelial membranes exhibit a KD of 0.88 nM and Bmax of 321.13 fmol mg-1 protein. Separation and purification of brush-border membranes yielded high affinity [125I]-AII binding sites with KD of 1.02 nM and Bmax of 56.6 fmol mg-1 protein. Angiotensin II receptors of the same affinity are present on renal cortex brush-border and basolateral membranes but a greater proportion are located on the latter. These receptors may be involved in the direct control of Na+ and water transport by AII.
摘要
血管紧张素II（AII）刺激钠负荷的肾皮质切片主动排出钠离子。部分纯化的基底外侧和刷状缘上皮膜中的特异性高亲和力[125I]-AII结合位点的解离常数（KD）为0.88 nM，最大结合容量（Bmax）为321.13 fmol/mg蛋白质。刷状缘膜的分离和纯化产生了高亲和力[125I]-AII结合位点，其KD为1.02 nM，Bmax为56.6 fmol/mg蛋白质。肾皮质刷状缘和基底外侧膜上存在相同亲和力的血管紧张素II受体，但后者上的受体比例更大。这些受体可能参与AII对钠和水转运的直接控制。
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本文引用的文献
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3
The binding of [125I]-angiotensin to rat renal epithelial cell membranes.[125I] -血管紧张素与大鼠肾上皮细胞膜的结合。
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