Cox H M, Munday K A, Poat J A
Br J Pharmacol. 1984 Aug;82(4):891-5. doi: 10.1111/j.1476-5381.1984.tb16487.x.
Angiotensin II (AII) stimulates active Na+ extrusion from Na+ loaded renal cortex slices. Specific high affinity [125I]-AII binding sites in partially purified basolateral and brush-border epithelial membranes exhibit a KD of 0.88 nM and Bmax of 321.13 fmol mg-1 protein. Separation and purification of brush-border membranes yielded high affinity [125I]-AII binding sites with KD of 1.02 nM and Bmax of 56.6 fmol mg-1 protein. Angiotensin II receptors of the same affinity are present on renal cortex brush-border and basolateral membranes but a greater proportion are located on the latter. These receptors may be involved in the direct control of Na+ and water transport by AII.
血管紧张素II(AII)刺激钠负荷的肾皮质切片主动排出钠离子。部分纯化的基底外侧和刷状缘上皮膜中的特异性高亲和力[125I]-AII结合位点的解离常数(KD)为0.88 nM,最大结合容量(Bmax)为321.13 fmol/mg蛋白质。刷状缘膜的分离和纯化产生了高亲和力[125I]-AII结合位点,其KD为1.02 nM,Bmax为56.6 fmol/mg蛋白质。肾皮质刷状缘和基底外侧膜上存在相同亲和力的血管紧张素II受体,但后者上的受体比例更大。这些受体可能参与AII对钠和水转运的直接控制。