Michalski F J
Yale J Biol Med. 1984 Sep-Oct;57(5):743-9.
Seven and one-half years of experience in a small diagnostic virology laboratory of a large inner-city hospital are reported. Seven hundred fifty-one viruses were isolated from over 8,000 specimens, using two types of tissue culture cells, human and monkey kidney. The most common isolates were Herpes simplex viruses (HSV) and Enteroviruses. Similar results have been reported by larger laboratories. Sensitivity for HSV in monkey kidney cells was only 75 percent that in human cells. An enzyme-linked immunosorbent assay (ELISA) for cytomegalovirus (CMV) was found to be a suitable substitute for the traditional complement fixation test (CF). IgM antibodies were not found in all HSV infections, but these antibodies did appear before CF antibodies in some cases. Monoclonal antibodies to HSV were effective in typing isolates, but for detection of viral antigen in brain smears of HSV encephalitis patients, polyclonal antibody gave better results.
本文报告了在一家大型市中心医院的小型诊断病毒学实验室七年半的工作经验。使用人肾和猴肾两种组织培养细胞,从8000多个标本中分离出751株病毒。最常见的分离株是单纯疱疹病毒(HSV)和肠道病毒。更大的实验室也报告了类似的结果。猴肾细胞中HSV的敏感性仅为人类细胞中的75%。发现一种针对巨细胞病毒(CMV)的酶联免疫吸附测定(ELISA)是传统补体结合试验(CF)的合适替代方法。并非所有HSV感染中都能检测到IgM抗体,但在某些情况下,这些抗体在CF抗体之前出现。针对HSV的单克隆抗体在分离株分型方面有效,但对于检测HSV脑炎患者脑涂片中的病毒抗原,多克隆抗体效果更好。