Cullum M E, Johnson B C, Zile M H
Int J Vitam Nutr Res. 1984;54(4):297-305.
Cellular retinol binding protein (CRBP) and cellular retinoic acid binding protein (CRABP) were assayed in bovine eye pigment epithelium (PE) cytosol from both early fetal and young adult animals. Both fetal and adult PE contain CRBP but only fetal PE contains CRABP. Blood retinol binding protein (RBP) was also assayed in serum from fetal and adult animals. Both fetal and adult serum contain RBP. A novel albumin adsorption technique (Affi-Gel Blue) combined with refinements of the sucrose gradient centrifugation technique provide a better assay method for retinoid receptors than polyacrylamide disc gel electrophoresis. Our findings support the hypothesis that retinoid receptors may cause or be the consequence of differentiation in animal tissues.
对来自早期胎儿和成年动物的牛眼色素上皮（PE）胞质溶胶中的细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）进行了测定。胎儿和成年PE均含有CRBP，但只有胎儿PE含有CRABP。还对胎儿和成年动物血清中的血液视黄醇结合蛋白（RBP）进行了测定。胎儿和成年血清均含有RBP。一种新型的白蛋白吸附技术（Affi-Gel Blue）与蔗糖梯度离心技术的改进相结合，为类视黄醇受体提供了一种比聚丙烯酰胺圆盘凝胶电泳更好的测定方法。我们的研究结果支持这样一种假设，即类视黄醇受体可能导致动物组织分化或为其分化的结果。
