高效液相色谱-双电化学检测法同时测定大鼠脑中鸟嘌呤核苷酸

Simultaneous determination of guanine nucleotides in rat brain by high-performance liquid chromatography with dual-electrochemical detection.

作者信息

Yamamoto T, Shimizu H, Kato T, Nagatsu T

出版信息

Anal Biochem. 1984 Nov 1;142(2):395-9. doi: 10.1016/0003-2697(84)90482-2.

DOI:10.1016/0003-2697(84)90482-2

Abstract

A new, sensitive, and specific assay method for guanine nucleotides using high-performance liquid chromatography with dual-electrochemical detection was developed. GTP, GDP, GMP, and cyclic GMP were separated with reversed-phase "ion-pair" chromatography and detected by a dual-electrochemical detector. Only guanine nucleotides among all purine and pyrimidine nucleotides responded to the electrochemical detector at 0.95 V. The peak heights for these guanine nucleotides were linear at concentrations between 0.5 pmol and 1 nmol. The regional distribution of these guanine nucleotides in the rat brain was studied by this new assay method.

摘要

开发了一种使用高效液相色谱双电化学检测法检测鸟嘌呤核苷酸的新的、灵敏且特异的测定方法。GTP、GDP、GMP和环磷酸鸟苷通过反相“离子对”色谱法分离，并由双电化学检测器检测。在所有嘌呤和嘧啶核苷酸中，只有鸟嘌呤核苷酸在0.95 V时对电化学检测器有响应。这些鸟嘌呤核苷酸的峰高在0.5 pmol至1 nmol的浓度范围内呈线性。用这种新的测定方法研究了这些鸟嘌呤核苷酸在大鼠脑中的区域分布。

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