Dunham B M, Hechtman H B, Valeri C R, Shepro D
Microcirc Endothelium Lymphatics. 1984 Aug;1(4):465-89.
One pathologic change common to the inflammatory process is loss of microvessel membrane integrity which results in edema. Polymorphonuclear leukocytes (PMN) are primary contributors to the development of edema because they cause tissue injury which alters vascular permeability and hemodynamics. The aim of this study was to assess the influence of arachidonic acid metabolites generated by activation of human PMN on the in vivo microvascular preparation of the hamster cheek pouch. Fluorescein-labeled dextran MW: 150,000 was used to assess microvascular permeability. Human PMN were activated with arachidonic acid (AA) and the calcium ionophore A23187, and the supernatant retained for testing. Topical application of the PMN supernatant, purified LTD4 or LTB4 resulted in marked extravasation of macromolecules from post-capillary venules of control hamsters. The extravasation was reduced when hamsters were pretreated with indomethacin (5 mg/kg), imidazole (25 mg/kg), ketoconazole (10 mg/kg), 13-azaprostanoic acid (30 mg/kg), FPL 55712 (1 mg/kg) and dimethylthiourea (500 mg/kg). The interpretation of the results suggests that the increased vascular permeability induced by PMN secretions may be mediated in part by the thromboxane pathway.
炎症过程中常见的一种病理变化是微血管膜完整性丧失，这会导致水肿。多形核白细胞（PMN）是水肿形成的主要促成因素，因为它们会造成组织损伤，进而改变血管通透性和血流动力学。本研究的目的是评估人PMN激活后产生的花生四烯酸代谢产物对仓鼠颊囊体内微血管制剂的影响。使用荧光素标记的葡聚糖（分子量：150,000）来评估微血管通透性。用人花生四烯酸（AA）和钙离子载体A23187激活人PMN，并保留上清液用于测试。局部应用PMN上清液、纯化的白三烯D4（LTD4）或白三烯B4（LTB4）会导致对照仓鼠毛细血管后微静脉出现明显的大分子外渗。当仓鼠用吲哚美辛（5毫克/千克）、咪唑（25毫克/千克）、酮康唑（10毫克/千克）、13-氮杂前列腺酸（30毫克/千克）、FPL 55712（1毫克/千克）和二甲基硫脲（500毫克/千克）预处理时，外渗减少。结果解释表明，PMN分泌物诱导的血管通透性增加可能部分由血栓素途径介导。
