Boucher R C, Bromberg P A, Gatzy J T
J Appl Physiol Respir Environ Exerc Physiol. 1980 Jan;48(1):169-76. doi: 10.1152/jappl.1980.48.1.169.
A technique for measurement of transmural bioelectric potential difference (PD) in vivo was adapted for use in mammalian airways. PD was measured between a Ringer-perfused exploring bridge positioned on the airway furface and a reference Ringer-agar bridge in the subcutaneous space. The in vivo PD of canine trachea from 50 dogs was 30.8 +/- 9.5 (SD) mV, lumen negative, indistinguishable from the peak in vitro transmural PD of the excised canine trachea. Liquid junction potentials between bridge perfusate and tracheal liquid were negligible. The response pattern to drugs applied to the mucosal surface in vivo was similar to that observed in vitro. The PD in canine bronchi 3-6 cm distal to the carina were substantially lower. Tracheal PDs of rabbit (-23 mV), guinea pig (-8 mV), and rat (-14 mV) were higher than bronchial PDs of the same species. PD across airway epithelia can be accurately and reliably measured in vivo and the lower bronchial PD suggests regional differences in electrolyte transport and/or permeability.
一种用于测量体内跨壁生物电位差（PD）的技术被应用于哺乳动物气道。在位于气道表面的林格液灌注探测桥与皮下空间的参比林格 - 琼脂桥之间测量PD。50只犬的犬气管体内PD为30.8±9.5（标准差）mV，管腔为负，与切除的犬气管的体外跨壁PD峰值无差异。桥灌注液与气管液体之间的液体接界电位可忽略不计。体内应用于粘膜表面的药物的反应模式与体外观察到的相似。隆突远端3 - 6 cm处的犬支气管中的PD显著更低。兔（-23 mV）、豚鼠（-8 mV）和大鼠（-14 mV）的气管PD高于同一物种的支气管PD。气道上皮的PD可以在体内准确可靠地测量，较低的支气管PD提示电解质转运和/或通透性存在区域差异。
