Wasada T, Howard B, Dobbs R E, Unger R H
J Clin Invest. 1980 Sep;66(3):511-6. doi: 10.1172/JCI109882.
To investigate the effect of acute elevation of plasma free fatty acids (FFA) on the secretion of splanchnic somatostatin-like immunoreactivity (SLI), the peripheral venous, pancreatic, and gastric venous effluent levels of SLI were measured in normal and chronic alloxan diabetic dogs before and after the infusion of a fat emulsion supplemented with heparin. In normal conscious dogs heparin injected during the infusion of a fat emulsion elevated FFA levels from a mean (+/-SE) base-line level of 0.7+/-0.1 meq/liter to a peak value of 1.5+/-0.1 meq/liter (P < 0.001) and plasma SLI rose from a mean (+/-SE) base-line value of 145+/-7 pg/ml to a peak of 253+/-44 pg/ml (P < 0.05). Neither the infusion of glycerol, of fat emulsion without heparin, of heparin alone nor of saline itself had an effect on either the plasma level of FFA or SLI. In another group of anesthetized dogs with surgically implanted catheters the administration of fat emulsion plus heparin was accompanied by more than a two-fold rise in the concentration of SLI in the venous effluent of the pancreas and of the gastric fundus and antrum in association with an elevation of FFA levels. In a group of conscious diabetic dogs fat emulsion plus heparin raised FFA from a mean base-line level of 1.2+/-0.2 to 1.6+/-0.3 meq/liter (P < 0.05) and SLI rose from a mean base-line level of 185+/-9 pg/ml to a peak value of 310+/-44 pg/ml (P < 0.01). Although SLI levels were significantly greater than in normal dogs at several time points after the rise in FFA, the magnitude of the increment in diabetic dogs did not differ from normal. These results demonstrate that a rise in FFA levels is a potent stimulus for SLI secretion from the pancreas and stomach and raise the possibility that FFA is an important physiological regulator of SLI secretion.
为研究血浆游离脂肪酸（FFA）急性升高对内脏生长抑素样免疫反应性（SLI）分泌的影响，在输注添加肝素的脂肪乳剂前后，测定了正常和慢性四氧嘧啶糖尿病犬外周静脉、胰腺及胃静脉流出液中SLI的水平。在正常清醒犬中，输注脂肪乳剂期间注射肝素使FFA水平从平均（±标准误）基线水平0.7±0.1毫当量/升升高至峰值1.5±0.1毫当量/升（P<0.001），血浆SLI从平均（±标准误）基线值145±7皮克/毫升升至峰值253±44皮克/毫升（P<0.05）。单独输注甘油、不添加肝素的脂肪乳剂、单独输注肝素或生理盐水本身，对FFA或SLI的血浆水平均无影响。在另一组通过手术植入导管的麻醉犬中，给予脂肪乳剂加肝素后，胰腺及胃底和胃窦静脉流出液中SLI浓度升高两倍多，同时FFA水平升高。在一组清醒糖尿病犬中，脂肪乳剂加肝素使FFA从平均基线水平1.2±0.2升高至1.6±0.3毫当量/升（P<0.05），SLI从平均基线水平185±9皮克/毫升升至峰值310±44皮克/毫升（P<0.01）。尽管在FFA升高后的几个时间点，糖尿病犬SLI水平显著高于正常犬，但糖尿病犬的升高幅度与正常犬并无差异。这些结果表明，FFA水平升高是胰腺和胃分泌SLI的有效刺激因素，并提示FFA可能是SLI分泌的重要生理调节因子。
