白三烯C和白三烯D生物合成的抑制作用。

Inhibition of leukotriene C and leukotriene D biosynthesis.

作者信息

Orning L, Hammarström S

出版信息

J Biol Chem. 1980 Sep 10;255(17):8023-6.

Abstract

Leukotriene C4 and leukotriene D4 are slow reacting substances produced by mouse mastocytoma cells and rat basophilic leukemia cells, respectively. Serine x borate complex, an inhibitor of glutamyl transpeptidase (EC 2.3.2.2), prevented the formation of the biologically more potent leukotriene D4 and caused accumulation of leukotriene C4 in rat basophilic leukemia cell suspensions. 5,8,11-Eicosatriynoic acid, a lipoxygenase inhibitor, prevented the biosynthesis of leukotriene C4 (ID50, 5 microM) by mastocytoma cells. The results demonstrate that gamma-glutamyl transpeptidase is involved in the biosynthesis of leukotriene D4 and suggest that leukotriene C4 is formed as an intermediate.

摘要

白三烯C4和白三烯D4分别是由小鼠肥大细胞瘤细胞和大鼠嗜碱性白血病细胞产生的慢反应物质。丝氨酸硼酸复合物是谷氨酰转肽酶（EC 2.3.2.2）的抑制剂，可阻止生物活性更强的白三烯D4的形成，并导致白三烯C4在大鼠嗜碱性白血病细胞悬液中积累。5,8,11-二十碳三烯酸是一种脂氧合酶抑制剂，可阻止肥大细胞瘤细胞合成白三烯C4（半数抑制浓度，5微摩尔）。结果表明，γ-谷氨酰转肽酶参与白三烯D4的生物合成，并提示白三烯C4是作为中间体形成的。