Pearce B R, Currie D N, Dutton G R, Hussey R E, Beale R, Pigott R
J Neurosci Methods. 1981 Feb;3(3):255-9. doi: 10.1016/0165-0270(81)90061-3.
A perfusion chamber is described for studying the efflux of putative neurotransmitters from CNS cells maintained in monolayer culture. We have used this apparatus to investigate the efflux of newly accumulated [3H]GABA from cell cultures of the early postnatal rat cerebellum.