利用人甲状腺膜的曲拉通增溶受体检测促甲状腺素结合抑制性免疫球蛋白

Detection of TSH-binding inhibitor immunoglobulins by using the triton-solubilized receptor from human thyroid membranes.

作者信息

Iida Y, Konishi J, Kasagi K, Kuma K, Torizuka K

出版信息

Endocrinol Jpn. 1982 Apr;29(2):227-31. doi: 10.1507/endocrj1954.29.227.

DOI:10.1507/endocrj1954.29.227

Abstract

A radioreceptor assay of TSH using Triton-solubilized human thyroid receptors was applied to the detection of TSH-binding inhibitor immunoglobulins (TBII). In 26 untreated patients with Graves' disease, 20 (76.9%) were found positive in this assay, while 18 (69.2%) cases were found positive by the conventional assay using human thyroid particulate receptors. In 25 patients with goitrous Hashimoto's thyroiditis, 2 (8.0%) were found positive by the assay using soluble receptor, while 3 (12.0%) were found positive by the conventional one. A significant correlation was found between TBII activities in the patients with untreated Graves' disease detected by these two assay systems (r=0.69; n=26; p less than 0.001). These data support the concept that TBII are antibodies against the TSH receptor or its closely related structures.

摘要

采用经 Triton 增溶的人甲状腺受体对促甲状腺激素（TSH）进行放射受体分析，用于检测 TSH 结合抑制性免疫球蛋白（TBII）。在 26 例未经治疗的格雷夫斯病患者中，该分析发现 20 例（76.9%）呈阳性，而使用人甲状腺微粒体受体的传统分析发现 18 例（69.2%）呈阳性。在 25 例甲状腺肿性桥本甲状腺炎患者中，使用可溶性受体的分析发现 2 例（8.0%）呈阳性，而传统分析发现 3 例（12.0%）呈阳性。这两种分析系统检测的未经治疗的格雷夫斯病患者的 TBII 活性之间存在显著相关性（r = 0.69；n = 26；p < 0.001）。这些数据支持 TBII 是针对 TSH 受体或其密切相关结构的抗体这一概念。