Ager A, Martin W
Br J Pharmacol. 1983 Sep;80(1):5-6. doi: 10.1111/j.1476-5381.1983.tb11040.x.
The responsiveness of freshly-isolated and subcultured pig aortic endothelial cells to adenosine triphosphate (ATP), bradykinin and ionophore A23187 was compared by monitoring agonist-induced 86Rb efflux. ATP, bradykinin and ionophore A23187 stimulated 86Rb efflux from freshly-isolated cells. ATP and bradykinin, which act via specific receptors, were less effective at inducing 86Rb efflux from subcultured cells but ionophore A23187 was as effective on subcultured as on freshly-isolated cells. These results suggest that pig aortic endothelial cells, when subcultured, lose receptors for ATP and bradykinin and/or develop an abnormality in the coupling-mechanism between receptor-occupation and calcium-mobilization.
通过监测激动剂诱导的86Rb外流,比较了新鲜分离和传代培养的猪主动脉内皮细胞对三磷酸腺苷(ATP)、缓激肽和离子载体A23187的反应性。ATP、缓激肽和离子载体A23187刺激了新鲜分离细胞的86Rb外流。通过特定受体起作用的ATP和缓激肽在诱导传代培养细胞的86Rb外流方面效果较差,但离子载体A23187对传代培养细胞和新鲜分离细胞的效果相同。这些结果表明,猪主动脉内皮细胞在传代培养时会失去ATP和缓激肽的受体,和/或在受体占据与钙动员之间的偶联机制中出现异常。