Inman M A, Butler M A, Connor T H, Matney T S
Teratog Carcinog Mutagen. 1983;3(6):491-501. doi: 10.1002/1520-6866(1990)3:6<491::aid-tcm1770030605>3.0.co;2-3.
Expansion of the Ames Salmonella/microsome mutagenesis test to include plasmid pKM101-bearing, excision repair-proficient derivatives permits 1) the identification of mutagens that require both factors for activity; 2) the identification of genotoxins through the enhancement of survival by excision repair; and 3) the classification of substances according to the effects of excision repair on their mutagenesis. Class I includes substances that require excision repair to effect mutagenesis. Class II contains substances whose mutagenesis is not affected by excision repair. Class III mutagens cause premutational lesions in DNA which are readily removed by excision repair. This classification scheme is suggested as a preliminary step in making a risk estimation for a mutagen.
将艾姆斯沙门氏菌/微粒体诱变试验扩展至包括携带质粒pKM101且切除修复功能正常的衍生物，可实现以下几点：1）识别需要这两种因素才能发挥活性的诱变剂；2）通过切除修复提高存活率来识别基因毒素；3）根据切除修复对其诱变作用的影响对物质进行分类。第一类包括需要切除修复才能产生诱变作用的物质。第二类包含诱变作用不受切除修复影响的物质。第三类诱变剂会在DNA中造成易于通过切除修复去除的前突变损伤。该分类方案被提议作为对诱变剂进行风险评估的初步步骤。
