酿酒酵母乙酰辅酶A合成酶的调控

Regulation of acetyl-CoA synthetase of Saccharomyces cerevisiae.

作者信息

Coleman J S, Bhattacharjee J K

出版信息

Can J Microbiol. 1976 May;22(5):762-4. doi: 10.1139/m76-110.

Abstract

Acetyl-coenzyme A synthetase (EC 6.2.1.1) activity of Saccharomyces cerevisiae was determined by a radioactive assay procedure. The activity in vitro was inhibited significantly by NADPH, NADH, or AMP and to a lesser extent by NADP, NAD, or ADP. Glutamic acid and alpha-ketoglutaric acid were not inhibitory. The enzyme level was repressed when the cells were grown in a complex nutrient medium as opposed to the minimal medium. However, a glutamic acid auxotroph glul, when grown in excess glutamic acid, demonstrated a fivefold increase of acetyl-CoA synthetase.

摘要

采用放射性检测方法测定了酿酒酵母的乙酰辅酶A合成酶（EC 6.2.1.1）活性。体外活性受到NADPH、NADH或AMP的显著抑制，受到NADP、NAD或ADP的抑制程度较小。谷氨酸和α-酮戊二酸没有抑制作用。与基本培养基相比，细胞在复杂营养培养基中生长时，该酶水平受到抑制。然而，谷氨酸营养缺陷型菌株glul在过量谷氨酸中生长时，乙酰辅酶A合成酶活性增加了五倍。