培养的PC12细胞中56 mM K⁺对酪氨酸羟化酶激活与磷酸化的关系。
Relationship between activation and phosphorylation of tyrosine hydroxylase by 56 mm K+ in PC12 cells in culture.
作者信息
Yanagihara N, Tank A W, Weiner N
出版信息
Mol Pharmacol. 1984 Jul;26(1):141-7.
Incubation of rat pheochromocytoma PC12 cells with 56 mM K+ is associated with increased activity and enhanced phosphorylation of tyrosine hydroxylase in the cells. The increase in the phosphorylation of tyrosine hydroxylase is observed after 30 sec of incubation with 56 mM K+; maximal phosphorylation is observed after 1 min of incubation. In contrast, although a significant increase in the activity of tyrosine hydroxylase is demonstrable after 30 sec of incubation with 56 mM K+, maximal activation is not attained until 3 min of incubation. Both the activation and increased phosphorylation of tyrosine hydroxylase exhibit a similar dependence upon potassium concentration in the incubation medium and are dependent on the presence of extracellular calcium. These results suggest that, although there is a relationship between activation and phosphorylation of tyrosine hydroxylase after potassium-evoked depolarization of rat pheochromocytoma PC12 cells in culture, this relationship may be complex.
将大鼠嗜铬细胞瘤PC12细胞与56 mM K⁺一起孵育，会使细胞中酪氨酸羟化酶的活性增加且磷酸化增强。在用56 mM K⁺孵育30秒后，可观察到酪氨酸羟化酶磷酸化增加；孵育1分钟后观察到最大磷酸化。相比之下，虽然在用56 mM K⁺孵育30秒后酪氨酸羟化酶活性有显著增加，但直到孵育3分钟才达到最大激活。酪氨酸羟化酶的激活和磷酸化增加对孵育培养基中的钾浓度表现出相似的依赖性，并且依赖于细胞外钙的存在。这些结果表明，尽管在培养的大鼠嗜铬细胞瘤PC12细胞经钾诱发去极化后，酪氨酸羟化酶的激活与磷酸化之间存在关联，但这种关系可能很复杂。
Zotero 插件