氯霉素处理的大肠杆菌中野生型和L8 - UV5 lac启动子的乳糖操纵子转录。
Lactose operon transcription from wild-type and L8-UV5 lac promoters in Escherichia coli treated with chloramphenicol.
作者信息
Hirschel B J, Shen V, Schlessinger D
出版信息
J Bacteriol. 1980 Sep;143(3):1534-7. doi: 10.1128/jb.143.3.1534-1537.1980.
Abstract
In cells treated with chloramphenicol and the inducer isopropyl-beta-D-thiogalacto-pyranoside, messenger ribonucleic acid transcription from the wild-type lac promoter was not detected. Transcription occurred from the mutant UV5-L8 promoter. The transcripts were of variable length; some included the whole Z gene. No major site of transcription arrest within the Z gene was apparent.
摘要
在用氯霉素和诱导剂异丙基-β-D-硫代半乳糖吡喃糖苷处理的细胞中，未检测到来自野生型乳糖启动子的信使核糖核酸转录。转录是从突变的UV5-L8启动子发生的。转录本长度不一；有些包含整个Z基因。在Z基因内没有明显的主要转录终止位点。
相似文献
1
Lactose operon transcription from wild-type and L8-UV5 lac promoters in Escherichia coli treated with chloramphenicol.
J Bacteriol. 1980 Sep;143(3):1534-7. doi: 10.1128/jb.143.3.1534-1537.1980.
2
lac Transcription in Escherichia coli cells treated with chloramphenicol.
J Bacteriol. 1982 Jul;151(1):251-61. doi: 10.1128/jb.151.1.251-261.1982.
3
Dual promoter control of the Escherichia coli lactose operon.
Cell. 1984 Nov;39(1):173-80. doi: 10.1016/0092-8674(84)90203-4.
4
The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585-9. doi: 10.1073/pnas.70.12.3585.
5
Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter.
J Mol Biol. 1984 Dec 25;180(4):881-909. doi: 10.1016/0022-2836(84)90262-6.
6
The tac promoter: a functional hybrid derived from the trp and lac promoters.
Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5. doi: 10.1073/pnas.80.1.21.
7
Lactose promoter mutation Pr115 activates an overlapping promoter within the lactose control region.
J Mol Biol. 1985 Oct 5;185(3):525-33. doi: 10.1016/0022-2836(85)90069-5.
8
Effects of Neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter. II. In vitro transcription analyses.
J Biol Chem. 1982 Nov 10;257(21):12962-9.
9
Mutational analysis of the lac regulatory region: second-site changes that activate mutant promoters.
Nucleic Acids Res. 1989 May 25;17(10):3909-25. doi: 10.1093/nar/17.10.3909.
10
Transcriptional slippage during the transcription initiation process at a mutant lac promoter in vivo.
J Mol Biol. 1993 Jun 5;231(3):569-80. doi: 10.1006/jmbi.1993.1310.
引用本文的文献
1
Highly tunable TetR-dependent target gene expression in the acetic acid bacterium Gluconobacter oxydans.
Appl Microbiol Biotechnol. 2021 Sep;105(18):6835-6852. doi: 10.1007/s00253-021-11473-x. Epub 2021 Aug 27.
2
Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems.
Nat Commun. 2021 Jan 12;12(1):325. doi: 10.1038/s41467-020-20094-3.
3
Autoregulation of Expression Relies on GraL Rather than on Promoter Region.
Int J Mol Sci. 2019 Oct 22;20(20):5224. doi: 10.3390/ijms20205224.
4
lac Transcription in Escherichia coli cells treated with chloramphenicol.
J Bacteriol. 1982 Jul;151(1):251-61. doi: 10.1128/jb.151.1.251-261.1982.
5
Regulation of cyclic AMP synthesis in Escherichia coli K-12: effects of the rpoD800 sigma mutation, glucose, and chloramphenicol.
J Bacteriol. 1984 Apr;158(1):110-4. doi: 10.1128/jb.158.1.110-114.1984.
本文引用的文献
1
Synthesis of Specific, Stabilized Messenger RNA When Translocation Is Blocked in ESCHERICHIA COLI.
Genetics. 1972 Feb;70(2):331-6. doi: 10.1093/genetics/70.2.331.
2
Catabolite-insensitive revertants of lac promoter mutants.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):773-9. doi: 10.1073/pnas.66.3.773.
3
New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
Virology. 1969 May;38(1):200-2. doi: 10.1016/0042-6822(69)90148-2.
4
Metabolism of messenger RNA from the gal operon of Escherichia coli.
J Mol Biol. 1974 Dec 15;90(3):581-99. doi: 10.1016/0022-2836(74)90236-8.
5
Diversity of regulation of genetic transcription. I. Effect of antibiotics which inhibit the process of translation on RNA metabolism in Escherichia coli.
J Mol Biol. 1973 Feb 25;74(2):113-36. doi: 10.1016/0022-2836(73)90102-2.
6
On the turnover of ppGpp in Escherichia coli.
J Biol Chem. 1972 Oct 10;247(19):6055-8.
7
Bearing of some recent results on the mechanisms of polarity and messenger RNA stability.
Mol Gen Genet. 1974;135(1):29-38. doi: 10.1007/BF00433898.
8
Diversity of regulation of genetic transcription. II. Specific relaxation of polarity in read-through transcription of the translocated trp operon in bacteriophage lambda trp.
J Mol Biol. 1974 Aug 25;87(4):741-54. doi: 10.1016/0022-2836(74)90082-5.
9
Effect of Rho on transcription of bacterial operons.
Nat New Biol. 1973 Feb 28;241(113):260-4. doi: 10.1038/newbio241260a0.
10
The effect of antibiotics on the in vivo synthesis of messenger ribonucleic acid from the lactose operon of Escherichia coli.
Mol Gen Genet. 1972;116(3):277-90. doi: 10.1007/BF00269771.
Zotero 插件