Robinson J, Sieff C, Delia D, Edwards P A, Greaves M
Nature. 1981 Jan 1;289(5793):68-71. doi: 10.1038/289068a0.
The unexpected discovery that Ia-like (HLA-DR) antigens in humans were present on blast cells from acute myeloblastic leukaemia led to the finding that normal granulocytic progenitors, in contrast to their mature descendents, also expressed HLA-DR antigens. Thus, anti-Ia sera stain a proportion of myeloblasts in normal bone marrow, inhibit myeloid progenitor (CFU-GM) colony formation in the presence of complement and can be used to label and separate CFU-GM on a fluorescence-activated cell sorter (FACS). Winchester et al. subsequently reported that erythroid progenitors (BFU-E and CFU-E) were also inhibited or killed by anti-Ia (p28,37) and complement. These observations raised the possibility that HLA-DR (or presumptive I-region equivalent) products might have a regulatory role in early haematopoiesis. We have now analysed HLA-DR and HLA-ABC antigen expression on normal erythroid progenitors using monoclonal antibodies to non-polymorphic determinants and fluorescence-activated cell sorting. In parallel experiments, we tested a monoclonal antibody to glycophorin, a well defined erythroid-specific cell-surface membrane glycoprotein. We report that HLA-DR, HLA-ABC and glycophorin are all expressed at various stages during erythroid differentiation.
人类急性髓细胞白血病原始细胞上存在Ia样（HLA - DR）抗原这一意外发现，导致了如下结果：与成熟后代相反，正常粒细胞祖细胞也表达HLA - DR抗原。因此，抗Ia血清可使正常骨髓中的一部分原始粒细胞着色，在有补体存在的情况下抑制髓系祖细胞（CFU - GM）集落形成，并且可用于在荧光激活细胞分选仪（FACS）上标记和分离CFU - GM。温彻斯特等人随后报道，红系祖细胞（BFU - E和CFU - E）也会被抗Ia（p28,37）和补体抑制或杀死。这些观察结果提出了一种可能性，即HLA - DR（或推测的I区等同物）产物可能在早期造血过程中具有调节作用。我们现在使用针对非多态性决定簇的单克隆抗体和荧光激活细胞分选技术，分析了正常红系祖细胞上HLA - DR和HLA - ABC抗原的表达情况。在平行实验中，我们测试了一种针对血型糖蛋白的单克隆抗体，血型糖蛋白是一种明确的红系特异性细胞表面膜糖蛋白。我们报道，HLA - DR、HLA - ABC和血型糖蛋白在红系分化的各个阶段均有表达。
