Perrier H, Llaurens G, Perrier C, Pérès G, Gras J
Rev Can Biol. 1980 Sep;39(3):141-7.
The levels of ornithine carbamyl transferase, acid phosphatase, alkaline phosphatase, leucine amino peptidase, creatine kinase, amylase, glutamic oxalacetic transaminase, glutamic pyruvic transaminase, lactate deshydrogenase, hydroxybutyrate deshydrogenase, glutamate deshydrogenase and malate deshydrogenase were determined in the plasma of Rainbow Trout and Tench submitted to water temperature increases. In the Rainbow Trout a thermal shock from 12 to 21 degrees, increases activities of some enzymes while temperature increase up to pre-mortem stage causes very important changes in enzymatic levels. In the Tench a thermal shock from 12 to 28 degrees causes more changes of enzymatic activities than a shock from 12 to 25 degrees. In Tench acclimated to 25 degrees, various enzyme levels are increased in comparison with 12 degrees control animals. A high potassium level in water causes complex changes in enzyme levels. The most sensitive enzymes to thermal disturbance are GOT and GPt transaminases which increase whatever the aggression form, amylase when thermal disturbance is moderate, alkaline phosphatase and malate deshydrogenase in case of strong thermal stress. The study of these enzymes is recommended for watching the state of fishes living in artificially heated waters.
测定了虹鳟和丁鱥在水温升高时血浆中鸟氨酸氨甲酰转移酶、酸性磷酸酶、碱性磷酸酶、亮氨酸氨基肽酶、肌酸激酶、淀粉酶、谷草转氨酶、谷丙转氨酶、乳酸脱氢酶、羟丁酸脱氢酶、谷氨酸脱氢酶和苹果酸脱氢酶的水平。在虹鳟中,从12℃到21℃的热休克会增加一些酶的活性,而温度升高到濒死阶段会导致酶水平发生非常重要的变化。在丁鱥中,从12℃到28℃的热休克比从12℃到25℃的热休克引起的酶活性变化更多。在适应25℃的丁鱥中,与12℃的对照动物相比,各种酶水平都有所升高。水中高钾水平会导致酶水平发生复杂变化。对热干扰最敏感的酶是谷草转氨酶和谷丙转氨酶,无论何种攻击形式它们都会升高;热干扰适中时淀粉酶升高;热应激强烈时碱性磷酸酶和苹果酸脱氢酶升高。建议通过研究这些酶来观察生活在人工加热水域中的鱼类的状态。