Skene J H, Willard M
J Neurochem. 1981 Jul;37(1):79-87. doi: 10.1111/j.1471-4159.1981.tb05293.x.
As a preliminary step to studying changes in axonal transport in regenerating neurons, we have analyzed the composition and organization of polypeptides normally axonally transported in a neuronal system capable of regeneration, i.e., the retinal ganglion cells of the toad, Bufo marinus. We labeled proteins synthesized in the retina with 35S-methionine and subsequently used one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis to analyze labeled, transported proteins in tissues containing segments of the axons (the optic nerve, optic tract, and optic tecta) of the retinal ganglion cells. The transported polypeptides could be divided into five groups according to their apparent transport velocities. Many of the polypeptides of each group were electrophoretically similar to polypeptides of corresponding groups previously described in rabbit and guinea pig retinal ganglion cells, and in some cases, additional properties of the polypeptides indicated that the transported materials of the two vertebrate classes were homologous. These results serve two purposes. First they establish the retinal ganglion cells of the toad Bufo marinus as a model system in which changes in gene expression related to regeneration may be studied. Second they show that the organization and many aspects of the composition of axonal transport in retinal ganglion cells have been conserved in animals as unrelated as amphibians, and mammals.
作为研究再生神经元轴突运输变化的初步步骤，我们分析了在一个能够再生的神经元系统（即海蟾蜍Bufo marinus的视网膜神经节细胞）中正常进行轴突运输的多肽的组成和组织。我们用35S-甲硫氨酸标记视网膜中合成的蛋白质，随后使用一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳来分析在含有视网膜神经节细胞轴突节段（视神经、视束和视顶盖）的组织中标记的、已运输的蛋白质。根据其明显的运输速度，运输的多肽可分为五组。每组中的许多多肽在电泳上与先前在兔和豚鼠视网膜神经节细胞中描述的相应组的多肽相似，并且在某些情况下，多肽的其他特性表明这两类脊椎动物运输的物质是同源的。这些结果有两个作用。第一，它们将海蟾蜍Bufo marinus的视网膜神经节细胞确立为一个模型系统，在其中可以研究与再生相关的基因表达变化。第二，它们表明，在像两栖动物和哺乳动物这样关系不紧密的动物中，视网膜神经节细胞轴突运输的组织和组成的许多方面是保守的。
